Supplementary Notes
ثبت نشده
چکیده
In a previous study in which we examined the genome-wide response of Arabidopsis to the xenobiotic compound trinitrotoluene (TNT) via cDNA microarrays, we found that Atwbc19 (At3g55130) was among upregulated genes. Given the role of ABC transporters in cellular detoxification we sought to further characterize this gene. Initial characterization of mutants revealed that root growth of Atwbc19 knockout mutants on media containing kanamycin was much slower than that of other ABC mutants. We specifically compared the kanamycin resistance level of mutants of Atwbc19 (SALK_107731) and two close homologues Atwbc18 (SALK_100187) and Atwbc16 (SALK_119868) that share over 76% similarity in amino acid sequence with Atwbc19 (see Supplementary Fig. 5). The mutants were T-DNA insertional mutants transformed with plasmid pROK2 containing an nptII gene, under the control of the nopaline synthase (nos) promoter. Therefore, we also compared their NPTII protein synthesis levels by ELISA assays. On media without kanamycin, there was no significant difference in root growth between the 3 lines. On media with kanamycin, root growth of line SALK_107731 was not different than that of SALK_119868 (0.31+0.04 and 0.30+0.03 cm, respectively) and clearly less than that of SALK_100187 (1.82+0.25 cm) as illustrated in Supplementary Figure 1. However, the amount of NPTII protein synthesized was more than 1000-fold higher in the SALK_107731 mutant compared to the other two mutants. Thus, these results strongly suggested that Atwbc19 had a role in kanamycin resistance while its two close homologues did not. The estimated level of resistance conferred by Atwbc19, under the control of its native promoter is equivalent to 1 μg NPTII per mg fresh weight, an
منابع مشابه
Robustness of Adaptive Expectations as an Equilibrium Selection Device – Supplementary
These are supplementary notes for Van Zandt and Lettau (2003). They include proofs of certain details in the main paper, and a few extensions. To be in synch, these notes and the main paper should have the same date.
متن کاملUnproductive splicing of SR genes associated with highly- and ultraconserved DNA elements
I. Supplementary methods and results ........................................................................S1 II. Supplementary figures (5) .......................................................................................S7 III. Supplementary tables (5)........................................................................................S12 IV. Supplementary notes (literature cited) ....
متن کاملInformation Overload in a Network of Targeted Communication: Supplementary Notes
These are supplementary notes for Van Zandt (2003). They include certain extensions. To be in synch, these notes and the main paper should have the same date. Author’s address: INSEAD Boulevard de Constance 77305 Fontainebleau CEDEX France Voice: +33 1 6072 4981 Fax: +33 1 6074 6192 Email: [email protected] Web: zandtwerk.insead.edu ∗I am indebted to Roy Radner and Peter Linhart for helpful d...
متن کاملSupplementary Notes: Inverse Kinematics Problems with Exact Hessian Matrices
These supplementary notes are provided in an educational spirit. Section 2 shows how the cross-product formula for computing columns of the Jacobian emerges from direct calculus. Section 5 extends to arbitrary Euler angles. Our work is not limited to homogeneous coordinates or Euler angles. However, these representations are well known and chosen merely to present to a wider audience. In fact o...
متن کاملSupplementary Notes for “Coalition Formation With Binding Agreements”
In these notes we provide some supplementary results and discussion not included in the main text of the paper. In particular, we provide a proof of the existence of a Markovian equilibrium, the proof of Proposition 3, some examples for three-player games and further discussion of the efficiency result. We also provide the proof of the four player inefficiency example and construct an equilibri...
متن کامل